{"id":128,"date":"2017-04-19T16:14:31","date_gmt":"2017-04-19T16:14:31","guid":{"rendered":"https:\/\/www.vanderbilt.edu\/csb\/?page_id=128"},"modified":"2025-11-05T16:31:57","modified_gmt":"2025-11-05T22:31:57","slug":"cryo-electron-microscopy","status":"publish","type":"page","link":"https:\/\/www.vanderbilt.edu\/csb\/facilities\/cryo-electron-microscopy\/","title":{"rendered":"CSB Cryo-Electron Microscopy Facility"},"content":{"rendered":"
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Ribosome<\/figcaption><\/figure>\n

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Facility Overview<\/h4>\n

The Center for Structural\u00a0Biology\u00a0Cryo-Electron Microscopy Facility\u00a0<\/b>houses\u00a0 cryo transmission electron microscopes for high resolution data collection of biological specimens by tomography and single-particle analysis.<\/p>\n

The Titan Krios G4<\/span> is the state-of-the-art 300kV microscope that combines equipment stability, enhanced automation and advanced software to aid researchers in efficient data collection. This microscope is equipped with a Volta phase plate, Gatan\u2019s BioQuantum energy filter<\/span>, K3 and Falcon3 direct electron detector<\/span> (DED) cameras and is computationally supported by multiple GPU workstations and a local 600TB RAID system. The Glacios<\/span> 200 kV microscope is equipped with a Falcon4i direct electron detector<\/span> and is capable of single particle, tomographic and MicroED applications.\u00a0 The L120C<\/span> 100kV TEM with Ceta-S 4k x 4k CMOS detector is used for screening and collecting images of negatively-stained specemins. The facility also has\u00a0two ThermoScientific Vitrobots<\/span>, one Leica EM GP2<\/span>, two carbon\/metal evaporators, glow discharge units and two Gatan 626<\/span> side-entry cryo sample holders.<\/p>\n

The microscopes are overseen by experienced staff who offer consultation and training in negative-stain and cryo sample preparation, single particle analysis, tomography, MicroED, data processing and additional computational support.<\/p>\n

Visit the facility iLab site<\/a> for equipment reservations, training and consultation requests. Click here<\/a> for information to set up an account for ACCRE and SBGrid.
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Mission Statement<\/h4>\n

The goal of\u00a0the\u00a0CSB Cryo-EM Facility<\/strong> is to make TEM accessible to all interested researchers at Vanderbilt. TEM is one of the fastest developing, cutting-edge structural techniques for exploring the organization of biological macromolecules. The unique strength of TEM is its versatility. Structures determined using TEM can span resolution ranges from very low- to atomic-levels (>30\u20131.8\u00c5) and do not require crystalline samples for analysis.<\/p>\n

In pursuit of its goal, the\u00a0CSB Cryo-EM Facility\u00a0<\/strong>enables TEM training and outreach by:<\/p>\n