Expression Vectors
CSB plasmids are freely available to Vanderbilt researchers. Contact Noah Saracho
for more information. The links for ‘Single Gene Expression’ require login with VUNet ID and password.
Single Gene Expression in E. coli:
| NT Tag(s) | Protease Cleavage Site | CT Tag(s) | Resistance | Vector |
|---|---|---|---|---|
| GST | TEV | His8 | Kan | pAT110 |
| 3C/PreScission | His8 | Kan | pAT109 | |
| His6 | Thrombin | ±á³§³Õ•H¾±²õ6 | Kan | pSV277 |
| TEV | ±á³§³Õ•H¾±²õ6 | Kan | pSV281 | |
| 3C/PreScission | ±á³§³Õ•H¾±²õ6 | Kan | pBG100 | |
| His6 | Amp | pHD116 | ||
| His10 | Thrombin | His8 | Kan | pNR114 |
| TEV | His8 | Kan | pNR111 | |
| 3C/PreScission | His8 | Kan | pBG106 | |
| ±á¾±²õ•G³§°Õ | TEV | ±á³§³Õ•H¾±²õ6 | Kan | pBG105 |
| 3C/PreScission | ±á³§³Õ•H¾±²õ6 | Kan | pBG101 | |
| ±á¾±²õ•Mµþ±Ê | Thrombin | ±á³§³Õ•H¾±²õ6 | Kan | pSV278 |
| TEV | ±á³§³Õ•H¾±²õ6 | Kan | pSV282 | |
| 3C/PreScission | ±á³§³Õ•H¾±²õ6 | Kan | pLM302 pLM303 |
|
| ±á¾±²õ•S±«²Ñ°¿ | TEV | ±á³§³Õ•H¾±²õ6 | Kan | pHD115 |
| 3C/PreScission | ±á³§³Õ•H¾±²õ6 | Kan | pBG102 pBG103 |
|
| Ulp1 | ±á³§³Õ•H¾±²õ6 | Kan | pBG104 | |
| MBP | TEV | His8 | Kan | pAT108 |
| 3C/PreScission | His8 | Kan | pAT107 | |
| °Õ°ù³æ•H¾±²õ | TEV | His6 | Amp | pNR113 |
| 3C/PreScission | ±á³§³Õ•H¾±²õ6 | Kan | pLM304 | |
| His6 | Amp | pNR112 |
Ìý
Multiple Gene Expression:
- Duet vectors for E. coli expression
- MultiColi vectors for E. coli expression
- MultiBac vectors for baculovirus expression
Membrane/Toxic Protein Expression
Recombinant Protease Expression:
Protocols:
Sequencing Primers